Eosin is a red stain and methylene blue is blue?

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Eosin is a red stain and methylene blue is blue. What should be the result of staining a bacterial smeal with a mixture of eosin and methylene blue?

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  1. Trevor H says:
    June 1, 2009 at 8:23 am

    Eosin is a fluorescent red dye resulting from the action of bromine on fluorescein. It can be used to stain cytoplasm, collagen and muscle fibers red for examination under the microscope.
    Methylene blue is used for totally different functions, such as RNA DNA examination.

    From my experience of using mixed stains to selectively colour mixtures of fibres, I would say that the Eosin would stain any cytoplasm, collagen and muscle fibers red and the methylene blue would stain other ingredients blue, or in a reducing medium, uncoloured.
    But I make no claims to being a microbiologist or pathologist, but I have had some experience of mixed microscopic stains in other spheres.

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